Licener is able to kill head lice and lice eggs (nits) in just one single application. Licener suffocates both by forming a coating that blocks the transport of oxygen. This is for a Family Size 200ml bottle of Licener. We also have a 100ml bottle available too in our online store.  Combing is not required to ensure the effectiveness of Licener. However if desired a nit comb can be used to remove the dead lice and nits after treatment. Licener is simple to use and works rapidly in only 10 minutes. The shampoo rinses out easily with no additional washing or combing required!